Journal: Acta Pharmaceutica Sinica. B

Article Title: Transfer learning enhanced graph neural network for aldehyde oxidase metabolism prediction and its experimental application

doi: 10.1016/j.apsb.2023.10.008

Figure Lengend Snippet: Identification of hAOX substrates using a liver cytosol assay (oxidative transformation).

Article Snippet: The human liver cytosol (mixed sex; a pool of 50 donors; catalog no. H0610.C, lot no. 1610027; 30 males and 20 females) was purchased from Sekisui XenoTech.

Techniques: Transformation Assay, Inhibition, Positive Control

Journal: Drug Metabolism and Disposition

Article Title: Stereospecific Metabolism of R - and S -Warfarin by Human Hepatic Cytosolic Reductases

doi: 10.1124/dmd.117.075929

Figure Lengend Snippet: Inhibitor phenotyping of steady-state reduction of R- and S-warfarin by HLC150. Reactions were carried out with two substrate concentrations, 50 µM (A and B) and 500 µM (C and D), 0.25 mg/ml protein, and 1 mM NADPH with inhibitors for specific cytosol reductases at 37°C and pH 7.4. (A and C) depict selective inhibition of R-warfarin to RS-warfarin alcohol. (B and D) show selective inhibition of S-warfarin to SS-warfarin alcohol. Reaction activities were normalized to the average control rate measured for reactions in the absence of inhibition. Rates were reported as percentages of the control rate. The reported activities are averaged from a minimum of five individual experiments ±S.E. Asterisks indicate statistical significance of inhibition compared with control as calculated using Student’s t test: *P < 0.05; **P < 0.005; ***P < 0.0005).

Article Snippet: High-performance liquid chromatography (HPLC)-grade methanol, acetic acid, dimethylsulfoxide (DMSO), and NADPH were purchased from Thermo Fisher (Pittsburgh, PA), and pooled human liver cytosol (HLC150) was from BD Biosciences (Franklin Lakes, NJ).

Techniques: Inhibition